Isolation and characterization of a bovine neural specific protein (CRMP-2) cDNA homologous to unc -33, a C. elegans gene implicated in axonal outgrowth and guidance
Identifieur interne : 001268 ( Istex/Checkpoint ); précédent : 001267; suivant : 001269Isolation and characterization of a bovine neural specific protein (CRMP-2) cDNA homologous to unc -33, a C. elegans gene implicated in axonal outgrowth and guidance
Auteurs : Tohru Kamata [États-Unis] ; Marianne Subleski [États-Unis] ; Yoshinobu Hara [États-Unis] ; Naoya Yuhki [États-Unis] ; Hsiang-Fu Kung [États-Unis] ; Neal G. Copeland [États-Unis] ; Nancy A. Jenkins [États-Unis] ; Teizo Yoshimura [États-Unis] ; William Modi [États-Unis] ; Terry D. Copeland [États-Unis]Source :
- Molecular Brain Research [ 0169-328X ] ; 1998.
English descriptors
- Teeft :
- Adult brain, Adult mice, Amino, Amino acid sequence, Amino acids, Assay, Astrocyte, Axon, Axonal, Axonal growth, Axonal guidance, Axonal outgrowth, Backcross, Bovine, Bovine brains, Bovine cdna, Caenorhabditis elegans, Cdna, Cdna encoding, Cdna fragment, Cdna library, Cell bodies, Cell body, Cells overexpressing, Central region, Cerebral cortex, Chromosome, Cingulate cortex, Clone, Coronal, Corpus callosum, Crmp, Crmp gene family, Dendrite, Early stage, Elegans, Elegans gene, Embryo, Embryonic, Embryonic brain, Encoding, Ganglion, Gene, Grml, Growth cone collapse, Growth cones, High levels, Homologue, Horse serum, Human chromosome, Human chromosomes, Hybridization, Immune complexes, Immunoblotting analysis, Immunoprecipitated, Immunoprecipitates, Kamata, Kinase, Kinase assay, Locus, Lysates, Membrane fractions, Molecular brain research, Mouse, Mouse chromosome, Mouse embryos, Mrna, Nervous system, Neural, Neural development, Neurite extension, Neuron, Neuronal, Neuronal axons, Neuronal cell bodies, Neuronal differentiation, Neuronal processes, Nucleotide, Oligodendrocyte, Other members, Outgrowth, Peptide, Phosphoamino acid analysis, Phosphoprotein, Phosphorylated, Phosphorylation, Phosphorylation level, Postmitotic neurons, Postnatal, Preimmune antibody, Protein, Protein kinases, Pyramidal cells, Rabbit antibody, Rna, Serine, Spinal cord, Spretus, Total rnas, Uncoordinated movement, Whole mouse embryo.
Abstract
Abstract: We have cloned the cDNA encoding bovine CRMP-2 from bovine brains. A full length cDNA encoding bovine CRMP-2 was isolated and sequenced. The deduced amino acid sequence reveals that the gene encodes a protein of 572 amino acids and is highly homologous to Caenorhabditis elegans unc-33, which controls the guidance and outgrowth of neuronal axons. The CRMP-2 transcript was present in bovine brains but not non-neural tissues, and its protein product existed in both soluble and membrane-bound forms. The expression of CRMP-2 protein and mRNA was upregulated during neuronal differentiation of rat PC12 cells. Immunoprecipitation of PC12 cell extracts shows that CRMP-2 was co-immunoprecipitated with a 190 kDa protein (p190). Both CRMP-2 and p190 were phosphorylated on serine residues in vivo and in vitro in a kinase assay of CRMP-2 immune complexes. Immunocytochemistry shows that CRMP-2 was exclusively localized in both the central and peripheral nervous systems in mouse embryos and detectable in the adult brain although the level of CRMP-2 decreased. The protein was expressed in the axon, dendrite, and cytoplasm of postmitotic neurons and in the cytoplasm of oligodendrocytes and astrocytes. The CRMP-2 gene maps to the region of mouse chromosome 14 syntenic with human chromosome 8p21. Taken together, these data suggest multi-functional roles for CRMP-2 in developing and adult nervous systems, and the biological activity of CRMP-2 could be regulated by phosphorylation reaction.
Url:
DOI: 10.1016/S0169-328X(97)00332-X
Affiliations:
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Copeland</name></author><author><name sortKey="Jenkins, Nancy A" sort="Jenkins, Nancy A" uniqKey="Jenkins N" first="Nancy A" last="Jenkins">Nancy A. Jenkins</name></author><author><name sortKey="Yoshimura, Teizo" sort="Yoshimura, Teizo" uniqKey="Yoshimura T" first="Teizo" last="Yoshimura">Teizo Yoshimura</name></author><author><name sortKey="Modi, William" sort="Modi, William" uniqKey="Modi W" first="William" last="Modi">William Modi</name></author><author><name sortKey="Copeland, Terry D" sort="Copeland, Terry D" uniqKey="Copeland T" first="Terry D" last="Copeland">Terry D. 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Copeland</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-FCRDC, Frederick, MD 21702</wicri:regionArea><wicri:noRegion>MD 21702</wicri:noRegion></affiliation></author><author><name sortKey="Jenkins, Nancy A" sort="Jenkins, Nancy A" uniqKey="Jenkins N" first="Nancy A" last="Jenkins">Nancy A. Jenkins</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-FCRDC, Frederick, MD 21702</wicri:regionArea><wicri:noRegion>MD 21702</wicri:noRegion></affiliation></author><author><name sortKey="Yoshimura, Teizo" sort="Yoshimura, Teizo" uniqKey="Yoshimura T" first="Teizo" last="Yoshimura">Teizo Yoshimura</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Immunopathology, NCI-FCRDC, Frederick, MD 21702</wicri:regionArea><wicri:noRegion>MD 21702</wicri:noRegion></affiliation></author><author><name sortKey="Modi, William" sort="Modi, William" uniqKey="Modi W" first="William" last="Modi">William Modi</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Viral Carcinogenesis, NCI-FCRDC, Frederick, MD 21702</wicri:regionArea><wicri:noRegion>MD 21702</wicri:noRegion></affiliation></author><author><name sortKey="Copeland, Terry D" sort="Copeland, Terry D" uniqKey="Copeland T" first="Terry D" last="Copeland">Terry D. Copeland</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Protein Chemistry, ABL-Basic Research Program, NCI-FCRDC, Frederick, MD 21702</wicri:regionArea><wicri:noRegion>MD 21702</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Brain Research</title><title level="j" type="abbrev">BRESM</title><idno type="ISSN">0169-328X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">54</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="219">219</biblScope><biblScope unit="page" to="236">236</biblScope></imprint><idno type="ISSN">0169-328X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0169-328X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adult brain</term><term>Adult mice</term><term>Amino</term><term>Amino acid sequence</term><term>Amino acids</term><term>Assay</term><term>Astrocyte</term><term>Axon</term><term>Axonal</term><term>Axonal growth</term><term>Axonal guidance</term><term>Axonal outgrowth</term><term>Backcross</term><term>Bovine</term><term>Bovine brains</term><term>Bovine cdna</term><term>Caenorhabditis elegans</term><term>Cdna</term><term>Cdna encoding</term><term>Cdna fragment</term><term>Cdna library</term><term>Cell bodies</term><term>Cell body</term><term>Cells overexpressing</term><term>Central region</term><term>Cerebral cortex</term><term>Chromosome</term><term>Cingulate cortex</term><term>Clone</term><term>Coronal</term><term>Corpus callosum</term><term>Crmp</term><term>Crmp gene family</term><term>Dendrite</term><term>Early stage</term><term>Elegans</term><term>Elegans gene</term><term>Embryo</term><term>Embryonic</term><term>Embryonic brain</term><term>Encoding</term><term>Ganglion</term><term>Gene</term><term>Grml</term><term>Growth cone collapse</term><term>Growth cones</term><term>High levels</term><term>Homologue</term><term>Horse serum</term><term>Human chromosome</term><term>Human chromosomes</term><term>Hybridization</term><term>Immune complexes</term><term>Immunoblotting analysis</term><term>Immunoprecipitated</term><term>Immunoprecipitates</term><term>Kamata</term><term>Kinase</term><term>Kinase assay</term><term>Locus</term><term>Lysates</term><term>Membrane fractions</term><term>Molecular brain research</term><term>Mouse</term><term>Mouse chromosome</term><term>Mouse embryos</term><term>Mrna</term><term>Nervous system</term><term>Neural</term><term>Neural development</term><term>Neurite extension</term><term>Neuron</term><term>Neuronal</term><term>Neuronal axons</term><term>Neuronal cell bodies</term><term>Neuronal differentiation</term><term>Neuronal processes</term><term>Nucleotide</term><term>Oligodendrocyte</term><term>Other members</term><term>Outgrowth</term><term>Peptide</term><term>Phosphoamino acid analysis</term><term>Phosphoprotein</term><term>Phosphorylated</term><term>Phosphorylation</term><term>Phosphorylation level</term><term>Postmitotic neurons</term><term>Postnatal</term><term>Preimmune antibody</term><term>Protein</term><term>Protein kinases</term><term>Pyramidal cells</term><term>Rabbit antibody</term><term>Rna</term><term>Serine</term><term>Spinal cord</term><term>Spretus</term><term>Total rnas</term><term>Uncoordinated movement</term><term>Whole mouse embryo</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have cloned the cDNA encoding bovine CRMP-2 from bovine brains. A full length cDNA encoding bovine CRMP-2 was isolated and sequenced. The deduced amino acid sequence reveals that the gene encodes a protein of 572 amino acids and is highly homologous to Caenorhabditis elegans unc-33, which controls the guidance and outgrowth of neuronal axons. The CRMP-2 transcript was present in bovine brains but not non-neural tissues, and its protein product existed in both soluble and membrane-bound forms. The expression of CRMP-2 protein and mRNA was upregulated during neuronal differentiation of rat PC12 cells. Immunoprecipitation of PC12 cell extracts shows that CRMP-2 was co-immunoprecipitated with a 190 kDa protein (p190). Both CRMP-2 and p190 were phosphorylated on serine residues in vivo and in vitro in a kinase assay of CRMP-2 immune complexes. Immunocytochemistry shows that CRMP-2 was exclusively localized in both the central and peripheral nervous systems in mouse embryos and detectable in the adult brain although the level of CRMP-2 decreased. The protein was expressed in the axon, dendrite, and cytoplasm of postmitotic neurons and in the cytoplasm of oligodendrocytes and astrocytes. The CRMP-2 gene maps to the region of mouse chromosome 14 syntenic with human chromosome 8p21. Taken together, these data suggest multi-functional roles for CRMP-2 in developing and adult nervous systems, and the biological activity of CRMP-2 could be regulated by phosphorylation reaction.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Kamata, Tohru" sort="Kamata, Tohru" uniqKey="Kamata T" first="Tohru" last="Kamata">Tohru Kamata</name></noRegion><name sortKey="Copeland, Neal G" sort="Copeland, Neal G" uniqKey="Copeland N" first="Neal G" last="Copeland">Neal G. Copeland</name><name sortKey="Copeland, Terry D" sort="Copeland, Terry D" uniqKey="Copeland T" first="Terry D" last="Copeland">Terry D. Copeland</name><name sortKey="Hara, Yoshinobu" sort="Hara, Yoshinobu" uniqKey="Hara Y" first="Yoshinobu" last="Hara">Yoshinobu Hara</name><name sortKey="Jenkins, Nancy A" sort="Jenkins, Nancy A" uniqKey="Jenkins N" first="Nancy A" last="Jenkins">Nancy A. Jenkins</name><name sortKey="Kung, Hsiang Fu" sort="Kung, Hsiang Fu" uniqKey="Kung H" first="Hsiang-Fu" last="Kung">Hsiang-Fu Kung</name><name sortKey="Modi, William" sort="Modi, William" uniqKey="Modi W" first="William" last="Modi">William Modi</name><name sortKey="Subleski, Marianne" sort="Subleski, Marianne" uniqKey="Subleski M" first="Marianne" last="Subleski">Marianne Subleski</name><name sortKey="Yoshimura, Teizo" sort="Yoshimura, Teizo" uniqKey="Yoshimura T" first="Teizo" last="Yoshimura">Teizo Yoshimura</name><name sortKey="Yuhki, Naoya" sort="Yuhki, Naoya" uniqKey="Yuhki N" first="Naoya" last="Yuhki">Naoya Yuhki</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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